Review

lif blocking antibody (R&D Systems)

Name: lif blocking antibody
Brand: R&D Systems
Rating: 91 (5 reviews)

R&D Systems is a verified supplier

R&D Systems manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

R&D Systems lif blocking antibody

Figure 1. Microﬂuidic chamber culture system. Mouse embryonic stem cells (mESCs) <t>were</t> <t>cultured</t> for 3 days in ES FBS media. (A): Fabricated PDMS device with the chamber ﬁlled with food dye, attached to a schematic showing mESC colonies within the chamber. Cloning cylinders atop the inlet and outlet of the microchamber serve as media reservoirs. (B): Hypothetical illustration showing mESCs maintain pluripotent phenotype (dome-shaped colony) in small volumes but differentiate (scattered phenotype) in large volumes. (C): Brightﬁeld images of mESCs in the presence and absence of exogenous <t>LIF.</t> The difference in phenotype is connected to the accumula- tion of endogenous signals in small volumes. Scale bar: 100 mm. Abbreviations: LIF, leukemia inhibitory factor; PDMS, Polydimethylsilox- ane; lC, microﬂuidic chamber.

Lif Blocking Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lif blocking antibody/product/R&D Systems
Average 91 stars, based on 5 article reviews

lif blocking antibody - by Bioz Stars, 2026-03

91/100 stars

Images

1) Product Images from "Embryonic Stem Cells Cultured in Microfluidic Chambers Take Control of Their Fate by Producing Endogenous Signals Including LIF."

Article Title: Embryonic Stem Cells Cultured in Microfluidic Chambers Take Control of Their Fate by Producing Endogenous Signals Including LIF.

Journal: Stem cells (Dayton, Ohio)

doi: 10.1002/stem.2324

Figure 1. Microﬂuidic chamber culture system. Mouse embryonic stem cells (mESCs) were cultured for 3 days in ES FBS media. (A): Fabricated PDMS device with the chamber ﬁlled with food dye, attached to a schematic showing mESC colonies within the chamber. Cloning cylinders atop the inlet and outlet of the microchamber serve as media reservoirs. (B): Hypothetical illustration showing mESCs maintain pluripotent phenotype (dome-shaped colony) in small volumes but differentiate (scattered phenotype) in large volumes. (C): Brightﬁeld images of mESCs in the presence and absence of exogenous LIF. The difference in phenotype is connected to the accumula- tion of endogenous signals in small volumes. Scale bar: 100 mm. Abbreviations: LIF, leukemia inhibitory factor; PDMS, Polydimethylsilox- ane; lC, microﬂuidic chamber.

Figure Legend Snippet: Figure 1. Microﬂuidic chamber culture system. Mouse embryonic stem cells (mESCs) were cultured for 3 days in ES FBS media. (A): Fabricated PDMS device with the chamber ﬁlled with food dye, attached to a schematic showing mESC colonies within the chamber. Cloning cylinders atop the inlet and outlet of the microchamber serve as media reservoirs. (B): Hypothetical illustration showing mESCs maintain pluripotent phenotype (dome-shaped colony) in small volumes but differentiate (scattered phenotype) in large volumes. (C): Brightﬁeld images of mESCs in the presence and absence of exogenous LIF. The difference in phenotype is connected to the accumula- tion of endogenous signals in small volumes. Scale bar: 100 mm. Abbreviations: LIF, leukemia inhibitory factor; PDMS, Polydimethylsilox- ane; lC, microﬂuidic chamber.

Techniques Used: Cell Culture, Cloning

Figure 2. Microchamber culture of mouse embryonic stem cells (mESCs) facilitates self-renewal in the absence of feeder cells and exogenous growth factors. mESCs were cultured for 3 days in ES FBS media. (A): mRNA expression levels of na€ıve and primed plu- ripotency markers analyzed by gene chip array. Fold increase is relative to values obtained for mESCs in standard tissue culture. (B): Relative mRNA expression level of pluripotency markers in mESCs cultured in standard tissue culture wells and microﬂuidic devices by qRT-PCR. Expression relative to mESCs harvested prior to seeding. (C): Immunoﬂuorescent images of cells in standard tis- sue culture wells and microchambers, stained for Oct3/4 (green) or Nanog (red) and counterstained with DAPI (blue). Scale bar: 100 mm. Abbreviations: LIF, leukemia inhibitory factor; lC, micro- chamber; qRT-PCR, quantitative reverse transcription polymerase chain reaction.

Figure Legend Snippet: Figure 2. Microchamber culture of mouse embryonic stem cells (mESCs) facilitates self-renewal in the absence of feeder cells and exogenous growth factors. mESCs were cultured for 3 days in ES FBS media. (A): mRNA expression levels of na€ıve and primed plu- ripotency markers analyzed by gene chip array. Fold increase is relative to values obtained for mESCs in standard tissue culture. (B): Relative mRNA expression level of pluripotency markers in mESCs cultured in standard tissue culture wells and microﬂuidic devices by qRT-PCR. Expression relative to mESCs harvested prior to seeding. (C): Immunoﬂuorescent images of cells in standard tis- sue culture wells and microchambers, stained for Oct3/4 (green) or Nanog (red) and counterstained with DAPI (blue). Scale bar: 100 mm. Abbreviations: LIF, leukemia inhibitory factor; lC, micro- chamber; qRT-PCR, quantitative reverse transcription polymerase chain reaction.

Techniques Used: Cell Culture, Expressing, Quantitative RT-PCR, Staining, Reverse Transcription, Polymerase Chain Reaction

Figure 4. Retention of pluripotency in microﬂuidic devices is a result of enhanced LIF/STAT3 signaling. (A): mRNA expression levels of endogenous ligands and receptors from microarray data. Fold increase is relative to values obtained for mouse embryonic stem cells (mESCs) cultured for 3 days in standard tissue culture with ES FBS media. (B): Expression of endogenous LIF in mESCs cultured for 3 days in standard tissue culture wells and microchambers without exogenous LIF supplementation. (C): Secretion of LIF from mESCs cul- tured in the absence of exogenous LIF (ELISA results are represented as mean 6 SD, n 5 3; p < .05). (D): Expression of pluripotency marker and direct LIF/STAT3 target gene KLF4, with or without a LIF blocking antibody. (E): Immunoﬂuorescent staining of phospho- STAT3 (Y705), in the presence and absence of exogenous LIF. Scale bar: 100 mm. Abbreviations: DAPI, 40,6-diamidino-2-phenylindole; KLF4, Kruppel-like factor 4; LIF, leukemia inhibitory factor; pSTAT3, phosphorylated (Y705) signal transducer and activator of transcription 3; STAT3, signal transducer and activator of transcription 3.

Figure Legend Snippet: Figure 4. Retention of pluripotency in microﬂuidic devices is a result of enhanced LIF/STAT3 signaling. (A): mRNA expression levels of endogenous ligands and receptors from microarray data. Fold increase is relative to values obtained for mouse embryonic stem cells (mESCs) cultured for 3 days in standard tissue culture with ES FBS media. (B): Expression of endogenous LIF in mESCs cultured for 3 days in standard tissue culture wells and microchambers without exogenous LIF supplementation. (C): Secretion of LIF from mESCs cul- tured in the absence of exogenous LIF (ELISA results are represented as mean 6 SD, n 5 3; p < .05). (D): Expression of pluripotency marker and direct LIF/STAT3 target gene KLF4, with or without a LIF blocking antibody. (E): Immunoﬂuorescent staining of phospho- STAT3 (Y705), in the presence and absence of exogenous LIF. Scale bar: 100 mm. Abbreviations: DAPI, 40,6-diamidino-2-phenylindole; KLF4, Kruppel-like factor 4; LIF, leukemia inhibitory factor; pSTAT3, phosphorylated (Y705) signal transducer and activator of transcription 3; STAT3, signal transducer and activator of transcription 3.

Techniques Used: Expressing, Microarray, Cell Culture, Enzyme-linked Immunosorbent Assay, Marker, Blocking Assay, Staining

Figure 5. Mouse embryonic stem cells (mESCs) inside mC maintain na€ıve pluripotency and JAK/STAT pathway activation in the absence of 2iL (CHIR99021, PD0325901, and LIF). mESCs were expanded inside mCs for 3 days without 2iL and compared to cells cultured in standard tissue culture plates in the presence and absence of 2iL. (A): Fluorescence images of mCherry and eGFP indicate miRNA expression proﬁles in 2iL or differentiation conditions (mCherry1/eGFP2, na€ıve pluripotency; mCherry1/eGFP1, postimplantation epiblast-like state). (B): Immunoﬂuorescent staining of phospho-STAT3 (Y705) in dual reporter mESCs after 48 hours of culture. (C): Line scan of ﬂuorescence intensity of phospho-STAT3 across the mESC colonies was analyzed by image J software. (D): Graph showing aver- age nuclear intensity of pSTAT3 from cells located in the center or periphery of hemispherical cell structures. Cells were chosen at ran- dom from ﬁve independent colonies (Data represents mean 6 SD, n 5 10; *p(interior) 5 .84, *p(periphery) 5 .00001). (E): Relative mRNA expression of LIF target genes in D3 mESCs cultured under standard and mC conditions. (F): When exposed to a Jak inhibitor (J1I) dual reporter mESCs in mCs differentiated rapidly as determined by morphology and coexpression of mir-290 and mir-302 on day 3 of cul- ture. Scale bar: 30 mm. Abbreviations: LIF, leukemia inhibitory factor; pSTAT3, phosphorylated (Y705) signal transducer and activator of transcription 3; lC, microchambers.

Figure Legend Snippet: Figure 5. Mouse embryonic stem cells (mESCs) inside mC maintain na€ıve pluripotency and JAK/STAT pathway activation in the absence of 2iL (CHIR99021, PD0325901, and LIF). mESCs were expanded inside mCs for 3 days without 2iL and compared to cells cultured in standard tissue culture plates in the presence and absence of 2iL. (A): Fluorescence images of mCherry and eGFP indicate miRNA expression proﬁles in 2iL or differentiation conditions (mCherry1/eGFP2, na€ıve pluripotency; mCherry1/eGFP1, postimplantation epiblast-like state). (B): Immunoﬂuorescent staining of phospho-STAT3 (Y705) in dual reporter mESCs after 48 hours of culture. (C): Line scan of ﬂuorescence intensity of phospho-STAT3 across the mESC colonies was analyzed by image J software. (D): Graph showing aver- age nuclear intensity of pSTAT3 from cells located in the center or periphery of hemispherical cell structures. Cells were chosen at ran- dom from ﬁve independent colonies (Data represents mean 6 SD, n 5 10; *p(interior) 5 .84, *p(periphery) 5 .00001). (E): Relative mRNA expression of LIF target genes in D3 mESCs cultured under standard and mC conditions. (F): When exposed to a Jak inhibitor (J1I) dual reporter mESCs in mCs differentiated rapidly as determined by morphology and coexpression of mir-290 and mir-302 on day 3 of cul- ture. Scale bar: 30 mm. Abbreviations: LIF, leukemia inhibitory factor; pSTAT3, phosphorylated (Y705) signal transducer and activator of transcription 3; lC, microchambers.

Techniques Used: Activation Assay, Cell Culture, Fluorescence, Expressing, Staining, Software

Figure 6. Subsequent BMP4 upregulation, following inhibition of gp130 ligand signaling, results in sequential differentiation of mouse embryonic stem cells (mESCs) into mesoderm. (A): Expression of na€ıve and primed pluripotency markers after 3 days of culture in micro- ﬂuidic devices, with or without a Jak1 inhibitor (J1I). (B): BMP4 expression following addition of a Jak1 inhibitor (J1I) reﬂects stage dependent expression as cells enter and exit EpiSC-like pluripotency. FGF5 and Brachyury were used as epiblast and mesoderm-lineage speciﬁc markers, respectively. (C, F): Brightﬁeld image of cells after 5 and 10 days of differentiation in microﬂuidic devices. (D): Expres- sion of BMP4, along with pluripotency (Oct3/4) and three germ layer markers (mesoderm: Brachyury, endoderm: FoxA2, and ectoderm: Sox1), after 5 days of culture under the indicated conditions. mESCs cultured in tissue culture plate under the same media conditions to that of the microchamber was used as standard. (E): Diagram describing media conditions that mESCs were exposed to over 10 days of differentiation. (G): Expression of mesoderm speciﬁc markers after 10 days of differentiation in microﬂuidic devices. J1I was used to induce differentiation for ﬁrst 5 days, while Dorsomorphin (Dorso) was used from 3 to 5 days of differentiation to block BMP4 signal pathway. (H): Schematic diagram showing the role of LIF in maintaining self-renewal and BMP4 in mesoderm differentiation. J1I acted to switch the fate of mESCs from their pluripotent state into a differentiation program. Scale bar: 50 mm. Abbreviations: BMP4, bone morphogenetic protein-4; EpiSC, epiblast stem cell; ESC, embryonic stem cell; LIF, leukemia inhibitory factor.

Figure Legend Snippet: Figure 6. Subsequent BMP4 upregulation, following inhibition of gp130 ligand signaling, results in sequential differentiation of mouse embryonic stem cells (mESCs) into mesoderm. (A): Expression of na€ıve and primed pluripotency markers after 3 days of culture in micro- ﬂuidic devices, with or without a Jak1 inhibitor (J1I). (B): BMP4 expression following addition of a Jak1 inhibitor (J1I) reﬂects stage dependent expression as cells enter and exit EpiSC-like pluripotency. FGF5 and Brachyury were used as epiblast and mesoderm-lineage speciﬁc markers, respectively. (C, F): Brightﬁeld image of cells after 5 and 10 days of differentiation in microﬂuidic devices. (D): Expres- sion of BMP4, along with pluripotency (Oct3/4) and three germ layer markers (mesoderm: Brachyury, endoderm: FoxA2, and ectoderm: Sox1), after 5 days of culture under the indicated conditions. mESCs cultured in tissue culture plate under the same media conditions to that of the microchamber was used as standard. (E): Diagram describing media conditions that mESCs were exposed to over 10 days of differentiation. (G): Expression of mesoderm speciﬁc markers after 10 days of differentiation in microﬂuidic devices. J1I was used to induce differentiation for ﬁrst 5 days, while Dorsomorphin (Dorso) was used from 3 to 5 days of differentiation to block BMP4 signal pathway. (H): Schematic diagram showing the role of LIF in maintaining self-renewal and BMP4 in mesoderm differentiation. J1I acted to switch the fate of mESCs from their pluripotent state into a differentiation program. Scale bar: 50 mm. Abbreviations: BMP4, bone morphogenetic protein-4; EpiSC, epiblast stem cell; ESC, embryonic stem cell; LIF, leukemia inhibitory factor.

Techniques Used: Inhibition, Expressing, Cell Culture, Blocking Assay

Image Search Results

Journal: Stem cells (Dayton, Ohio)

Article Title: Embryonic Stem Cells Cultured in Microfluidic Chambers Take Control of Their Fate by Producing Endogenous Signals Including LIF.

doi: 10.1002/stem.2324

Figure Lengend Snippet: Figure 1. Microﬂuidic chamber culture system. Mouse embryonic stem cells (mESCs) were cultured for 3 days in ES FBS media. (A): Fabricated PDMS device with the chamber ﬁlled with food dye, attached to a schematic showing mESC colonies within the chamber. Cloning cylinders atop the inlet and outlet of the microchamber serve as media reservoirs. (B): Hypothetical illustration showing mESCs maintain pluripotent phenotype (dome-shaped colony) in small volumes but differentiate (scattered phenotype) in large volumes. (C): Brightﬁeld images of mESCs in the presence and absence of exogenous LIF. The difference in phenotype is connected to the accumula- tion of endogenous signals in small volumes. Scale bar: 100 mm. Abbreviations: LIF, leukemia inhibitory factor; PDMS, Polydimethylsilox- ane; lC, microﬂuidic chamber.

Article Snippet: Subsequently, cells were cultured in media without LIF or containing a LIF blocking antibody (500 ng/ml; R&D Systems, Minneapolis, MN, https://www.rndsystems.com) for 48 hours with daily media exchange.

Techniques: Cell Culture, Cloning

Journal: Stem cells (Dayton, Ohio)

Article Title: Embryonic Stem Cells Cultured in Microfluidic Chambers Take Control of Their Fate by Producing Endogenous Signals Including LIF.

doi: 10.1002/stem.2324

Figure Lengend Snippet: Figure 2. Microchamber culture of mouse embryonic stem cells (mESCs) facilitates self-renewal in the absence of feeder cells and exogenous growth factors. mESCs were cultured for 3 days in ES FBS media. (A): mRNA expression levels of na€ıve and primed plu- ripotency markers analyzed by gene chip array. Fold increase is relative to values obtained for mESCs in standard tissue culture. (B): Relative mRNA expression level of pluripotency markers in mESCs cultured in standard tissue culture wells and microﬂuidic devices by qRT-PCR. Expression relative to mESCs harvested prior to seeding. (C): Immunoﬂuorescent images of cells in standard tis- sue culture wells and microchambers, stained for Oct3/4 (green) or Nanog (red) and counterstained with DAPI (blue). Scale bar: 100 mm. Abbreviations: LIF, leukemia inhibitory factor; lC, micro- chamber; qRT-PCR, quantitative reverse transcription polymerase chain reaction.

Techniques: Cell Culture, Expressing, Quantitative RT-PCR, Staining, Reverse Transcription, Polymerase Chain Reaction

Journal: Stem cells (Dayton, Ohio)

Article Title: Embryonic Stem Cells Cultured in Microfluidic Chambers Take Control of Their Fate by Producing Endogenous Signals Including LIF.

doi: 10.1002/stem.2324

Figure Lengend Snippet: Figure 4. Retention of pluripotency in microﬂuidic devices is a result of enhanced LIF/STAT3 signaling. (A): mRNA expression levels of endogenous ligands and receptors from microarray data. Fold increase is relative to values obtained for mouse embryonic stem cells (mESCs) cultured for 3 days in standard tissue culture with ES FBS media. (B): Expression of endogenous LIF in mESCs cultured for 3 days in standard tissue culture wells and microchambers without exogenous LIF supplementation. (C): Secretion of LIF from mESCs cul- tured in the absence of exogenous LIF (ELISA results are represented as mean 6 SD, n 5 3; p < .05). (D): Expression of pluripotency marker and direct LIF/STAT3 target gene KLF4, with or without a LIF blocking antibody. (E): Immunoﬂuorescent staining of phospho- STAT3 (Y705), in the presence and absence of exogenous LIF. Scale bar: 100 mm. Abbreviations: DAPI, 40,6-diamidino-2-phenylindole; KLF4, Kruppel-like factor 4; LIF, leukemia inhibitory factor; pSTAT3, phosphorylated (Y705) signal transducer and activator of transcription 3; STAT3, signal transducer and activator of transcription 3.

Techniques: Expressing, Microarray, Cell Culture, Enzyme-linked Immunosorbent Assay, Marker, Blocking Assay, Staining

Journal: Stem cells (Dayton, Ohio)

Article Title: Embryonic Stem Cells Cultured in Microfluidic Chambers Take Control of Their Fate by Producing Endogenous Signals Including LIF.

doi: 10.1002/stem.2324

Figure Lengend Snippet: Figure 5. Mouse embryonic stem cells (mESCs) inside mC maintain na€ıve pluripotency and JAK/STAT pathway activation in the absence of 2iL (CHIR99021, PD0325901, and LIF). mESCs were expanded inside mCs for 3 days without 2iL and compared to cells cultured in standard tissue culture plates in the presence and absence of 2iL. (A): Fluorescence images of mCherry and eGFP indicate miRNA expression proﬁles in 2iL or differentiation conditions (mCherry1/eGFP2, na€ıve pluripotency; mCherry1/eGFP1, postimplantation epiblast-like state). (B): Immunoﬂuorescent staining of phospho-STAT3 (Y705) in dual reporter mESCs after 48 hours of culture. (C): Line scan of ﬂuorescence intensity of phospho-STAT3 across the mESC colonies was analyzed by image J software. (D): Graph showing aver- age nuclear intensity of pSTAT3 from cells located in the center or periphery of hemispherical cell structures. Cells were chosen at ran- dom from ﬁve independent colonies (Data represents mean 6 SD, n 5 10; *p(interior) 5 .84, *p(periphery) 5 .00001). (E): Relative mRNA expression of LIF target genes in D3 mESCs cultured under standard and mC conditions. (F): When exposed to a Jak inhibitor (J1I) dual reporter mESCs in mCs differentiated rapidly as determined by morphology and coexpression of mir-290 and mir-302 on day 3 of cul- ture. Scale bar: 30 mm. Abbreviations: LIF, leukemia inhibitory factor; pSTAT3, phosphorylated (Y705) signal transducer and activator of transcription 3; lC, microchambers.

Techniques: Activation Assay, Cell Culture, Fluorescence, Expressing, Staining, Software

Journal: Stem cells (Dayton, Ohio)

Article Title: Embryonic Stem Cells Cultured in Microfluidic Chambers Take Control of Their Fate by Producing Endogenous Signals Including LIF.

doi: 10.1002/stem.2324

Figure Lengend Snippet: Figure 6. Subsequent BMP4 upregulation, following inhibition of gp130 ligand signaling, results in sequential differentiation of mouse embryonic stem cells (mESCs) into mesoderm. (A): Expression of na€ıve and primed pluripotency markers after 3 days of culture in micro- ﬂuidic devices, with or without a Jak1 inhibitor (J1I). (B): BMP4 expression following addition of a Jak1 inhibitor (J1I) reﬂects stage dependent expression as cells enter and exit EpiSC-like pluripotency. FGF5 and Brachyury were used as epiblast and mesoderm-lineage speciﬁc markers, respectively. (C, F): Brightﬁeld image of cells after 5 and 10 days of differentiation in microﬂuidic devices. (D): Expres- sion of BMP4, along with pluripotency (Oct3/4) and three germ layer markers (mesoderm: Brachyury, endoderm: FoxA2, and ectoderm: Sox1), after 5 days of culture under the indicated conditions. mESCs cultured in tissue culture plate under the same media conditions to that of the microchamber was used as standard. (E): Diagram describing media conditions that mESCs were exposed to over 10 days of differentiation. (G): Expression of mesoderm speciﬁc markers after 10 days of differentiation in microﬂuidic devices. J1I was used to induce differentiation for ﬁrst 5 days, while Dorsomorphin (Dorso) was used from 3 to 5 days of differentiation to block BMP4 signal pathway. (H): Schematic diagram showing the role of LIF in maintaining self-renewal and BMP4 in mesoderm differentiation. J1I acted to switch the fate of mESCs from their pluripotent state into a differentiation program. Scale bar: 50 mm. Abbreviations: BMP4, bone morphogenetic protein-4; EpiSC, epiblast stem cell; ESC, embryonic stem cell; LIF, leukemia inhibitory factor.

Techniques: Inhibition, Expressing, Cell Culture, Blocking Assay

Cytokines/growth factors released into the conditioned medium of hAFSCs and MSCs and effects on tubular cell proliferation. A: Evaluation of cytokines/growth factors released into the conditioned medium of 1 × 106 hAFSCs and MSCs after a 12-hour incubation in RPMI 1640 plus 0.5% bovine serum albumin. Cytokines were measured using a multiplex cytokine array. Data represent the mean ± SD of five different cell lines. FGF, fibroblast growth factor; PDGF, platelet-derived growth factor; VEGF, vascular endothelial growth factor; LIF, leukemia inhibitory factor; NGF, nerve growth factor; SCF, stem cell factor; SDF, stromal derived factor; HGF, hepatocyte growth factor. B: Proliferation of TECs induced by the conditioned medium (CM) of hAFSCs and MSCs was evaluated by incorporation of BrdU and expressed as the percent increase over unstimulated control cells (Ctrl). The role of LIF in proliferation induced by the conditioned medium was evaluated using 3 μg/ml anti-LIF blocking antibodies (Ab-LIF). Data represent the mean ± SD of three different experiments performed in duplicate. Analysis of variance with Dunnett's comparison test: *P < 0.005, CM plus Ab-LIF versus CM alone.

Journal: The American Journal of Pathology

Article Title: Stem Cells Derived from Human Amniotic Fluid Contribute to Acute Kidney Injury Recovery

doi: 10.2353/ajpath.2010.091245

Figure Lengend Snippet: Cytokines/growth factors released into the conditioned medium of hAFSCs and MSCs and effects on tubular cell proliferation. A: Evaluation of cytokines/growth factors released into the conditioned medium of 1 × 106 hAFSCs and MSCs after a 12-hour incubation in RPMI 1640 plus 0.5% bovine serum albumin. Cytokines were measured using a multiplex cytokine array. Data represent the mean ± SD of five different cell lines. FGF, fibroblast growth factor; PDGF, platelet-derived growth factor; VEGF, vascular endothelial growth factor; LIF, leukemia inhibitory factor; NGF, nerve growth factor; SCF, stem cell factor; SDF, stromal derived factor; HGF, hepatocyte growth factor. B: Proliferation of TECs induced by the conditioned medium (CM) of hAFSCs and MSCs was evaluated by incorporation of BrdU and expressed as the percent increase over unstimulated control cells (Ctrl). The role of LIF in proliferation induced by the conditioned medium was evaluated using 3 μg/ml anti-LIF blocking antibodies (Ab-LIF). Data represent the mean ± SD of three different experiments performed in duplicate. Analysis of variance with Dunnett's comparison test: *P < 0.005, CM plus Ab-LIF versus CM alone.

Article Snippet: 29 The cells were seeded at 4000 cells/well into 96-well plates in Dulbecco's modified Eagle's medium (Sigma-Aldrich), deprived of fetal calf serum incubated with or without 3 μg/ml of human leukemia inhibitory factor (LIF) blocking antibody (R&D Systems) in the presence of 30% conditioned medium from hAFSCs or MSCs.

Techniques: Incubation, Multiplex Assay, Derivative Assay, Blocking Assay, Comparison

Review

Structured Review

Images

1) Product Images from "Embryonic Stem Cells Cultured in Microfluidic Chambers Take Control of Their Fate by Producing Endogenous Signals Including LIF."

Similar Products

Image Search Results